【摘要】 应用PCR方法克隆了香蕉束顶病毒中国漳州分离物 (BananabunchytopvirusChineseZhangzhouisolate ,BBTV_ZZ)DNA 4。序列分析表明其序列全长为 10 39nt,归属于亚洲组。 5′RACE分析确定其转录起始位点是 2 6 9nt处的A。利用PCR方法亚克隆了BBTV_ZZDNA 4非编码区序列并将其插入到植物表达载体pCAMBIA 130 4中的gfp∶∶gus基因上游得到重组质粒pTA2。将含pTA2和pCAMBIA 130 4的根癌土壤杆菌 (Agrobacteriumtumefaciens)注射进烟草 (NicotianatabacumL .cv .XanthiNC)叶片 ,3～ 5d后剪下注射部位的叶片进行GUS和GFP的表达分析。pTA2 (含BBTV_ZZDNA 4非编码区 )、pCAMBIA 130 4 (含CaMV 35S启动子 )和未注射的烟草叶片的GUS活性分别为 1 0 0 70pmolMU·μg-1·min-1,2 .0 6 90pmolMU·μg-1·min-1和 0 .0 2 14pmolMU·μg-1·min-1。注射含pTA2和pCAMBIA 130 4植物表达载体根癌土壤杆菌以及未注射的烟草叶片的每毫克总蛋白的GFP间接ELISA在 4 90nm的吸光值分别为89 5 77、10 0 4 4 0和 3 2 87。更多还原

【Abstract】 Banana bunchy top virus Chinese Zhangzhou isolate (BBTV_ZZ) DNA 4 was amplified by PCR and cloned. Sequence analysis showed that BBTV_ZZ DNA 4 is 1 039 nucleotides (nts) in length and this virus could be one member of BBTV Asian group. Transcriptional initiation site A, which is at the 269 nucleotide, was preliminarily determined by using 5′ RACE method. BBTV_ZZ DNA 4 non_coding region was sub_cloned by PCR and inserted into upstream of gfp∶∶gus plant expression vector pCAMBIA 1304 to construct recombinant plasmid pTA2. Agrobacterium tumefaciens harboring pTA2 was injected into leaves of the tobacco ( Nicotiana tabacum L. cv. Xanthi NC) via Agrobacterium _infiltration procedure. Transient expressions of GUS and GFP were determined in injected leaves 3-5 d later. GUS activities of pTA2, pCAMBIA 1304 injected and non_injected tobacco leaves respectively were 1.007 0 pmol MU·μg -1 ·min -1 , 2.069 0 pmol MU·μg -1 ·min -1 and 0.021 4 pmol MU·μg -1 ·min -1 . Indirect ELISA for GFP in 1 mg total protein from pTA2, pCAMBIA 1304 injected and non_injected leaves showed an A 490 nm value of 89.577, 100.440 and 3.287, respectively. These results showed that the non_coding region of BBTV_ZZ DNA 4 has a promoter activity not only in the virus replication in monocot, but also in driving the expression of a foreign gene in dicot plants.更多还原