【摘要】 用 SPF鸡胚从发生新城疫鸡群分离到一株病毒 ,进行了生物学特性的研究 ,结果表明 ,最小致死量鸡胚平均死亡时间 (MDT)为 5 0 ,1日龄鸡脑内接种致病指数 (ICPI)为 1.82 ,6周龄鸡静脉接种致病指数 (IVPI)为 2 .6 2 ,属强毒力毒株。以异硫氰酸胍法提取病毒基因组 RNA,RT- PCR扩增其融合蛋白 F基因重要功能区片段 ,经回收后 ,克隆到 p GEM- T载体上进行酶切鉴定与核苷酸序列测定 ,并与多株已报道的 NDV国内外参考株相应片段进行序列比较 ,F基因的同源性在 84% - 91%之间 ,氨基酸同源性为 82 % - 93% ,经基因进化树分析 ,证实流行株为基因 型毒株更多还原

【Abstract】 A NDV strain was isolated using SPF chicken embryos.Its biological characteristics were studied.The results showed that the NDV strain was velogenic and the MDT,the ICPI of one-day-old chicken,the IVPI of six-week-old chicken were respectively 50,1.82 and 2.62.For preparation of RNA,the acid guanidiniu-thiocynate method was used,F gene was amplified by RT-PCR and cloned into pGEM-T vector.The cDNA was sequenced by sanger’s method.Compared with the corresponding fragment of NDV strains that have been reported. We concluded analysis using phylogenetic tree of NDV strains indicated that the NDV strain isolated from Shandong belonged to genetype VII.更多还原