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SPA直接平板协同凝集试验诊断鸡传染性法氏囊病
Diagnosis of Infectious Bursal Disease of Chicken Using the Direct Plate Co-agglutination Test of SPA of Chicken Origin
【摘要】 自葡萄球菌病鸡分离到的 8株金黄色葡萄球菌中 ,筛选出 1株富含 SPA的菌株 ,在此基础上 ,建立了诊断鸡传染性法氏囊病 (IBD)的 SPA直接平板协同凝集试验。经特异性检验、敏感性试验及临床应用表明 ,该法具有敏感性高、特异性强、重复性好等优点 ,与琼脂扩散试验及对流免疫电泳试验比较 ,3者间无明显差异 ,且操作方法简便、快速 ,节省材料 ,是一种 IBD早期病原诊断及流行病学调查的新技术。
【Abstract】 Eight strains of Staphylococcus aureus of chicken staphylococcosis origin were tested for their ability to bind chicken serum.One of isolates(CS 6 strain) produced high amounts of staphylococcal protein A(SPA) and showed the reactivity to chicken serum.On the basis of this,a SPA direct plate co-agglutination test used in diagnosis of infectious bursal disease of chickens has been firstly established which has the advantage of simple,quick,specibility,reproducibility and sensitive.
【关键词】 葡萄球菌蛋白A;
直接平板协同凝集试验;
鸡;
传染性法氏囊病;
【Key words】 staphylococcal protein A; direct plate coagglutination test; chicken; infectious bursal disease;
【Key words】 staphylococcal protein A; direct plate coagglutination test; chicken; infectious bursal disease;
- 【文献出处】 中国兽医学报 ,Chinese Journal of Veterinary Science , 编辑部邮箱 ,2002年02期
- 【分类号】S858.31
- 【被引频次】16
- 【下载频次】119