【摘要】 目的 探讨丹参(Radix saliviae miltiorrizae,RSM)在肝纤维化过程中的作用。方法 采用大鼠腹腔注射硫代乙酰胺(TAA),复制肝纤维化模型。分别给不同组大鼠腹腔注射TAA和皮下注射丹参,13周后观察谷丙氨酸转氨酶(ALT)、乳酸脱氢酶(LDH)、血浆内毒素LPs含量、血浆和肝组织NO含量、HE和V·G染色观察各组肝组织形态改变。结果 TAA组:ALT、LDH、LPS羟脯氨酸、血浆和肝组织NO含量均高于正常对照组(P<0.05),丹参组上述指标均低于TAA组,组织学观察丹参组肝损伤和纤维化程度较TAA组轻。结论 丹参在TAA诱导的肝纤维化过程中有保护作用。更多还原

【Abstract】 Objective The purpose of this study was to investigate the role of RSM in experimental hepatic fibrosis. Methods Experimental hepatic fibrosis in rats were induced by injecting of thioacetamide in troperitioneally. Rats were divided into normal control group、TAA group and RSM group. TAA group and RSM group rats were injected TAA (100 mg/kg ip) and RSM (1ml sc) . After 13 weeks. Plasma endotoxin content、serum alanine transa minase (ALT) serum LDH activity and plasma nitric oxide (NO) and hepatic tissue NO、collagen were measured. The changes of hepatic histology were observed by HE and V’G stain Results Liver injury and liver fibrosis generated in rats with TAA (ip) . The results indicated that ALT、LDH、LPS、collagen and NO contents in plasma and hepatic tissue NO contents were increased significantly in TAA group (vs control group P < 0.05) .those indices were lower in RSM group than those in TAA group. HE and V·G stain showed that the grade of hepatic fibrosis and hepatic injury were more serious in TAA group than those in RSM group. Conclusion RSM play a protective role in the process of hepatic fibrosis.更多还原