【摘要】 目的　探讨应用PCR技术对药物治疗小鼠急性弓形虫感染的疗效进行了考核评价。方法　小鼠腹腔内感染RH株弓形虫速殖子 2× 10 3 ,8h后给予双氢青蒿素 75mg/kg d和双氢青蒿素 75mg/kg d联合磺胺嘧啶钠 10 0mg/kg d每日两次灌胃小鼠治疗 ,疗程 15d ,观察小鼠存活率 ,并于感染后第 9、15、31d取小鼠腹水及肝、脾和脑进行弓形虫PCR检测 ;于停药后5个月取存活小鼠脑再行弓形虫PCR检测。结果　双氢青蒿素联合磺胺嘧啶钠组小鼠存活率达 93% ,明显高于磺胺嘧啶钠组 (6 8 3% )及双氢青蒿素组 (0 % )。PCR检测可见小鼠腹水中除联合治疗组外 ,其余各组均可检测出特异弓形虫DNA ;但只有对照组和双氢青蒿素组小鼠的肝脏和脑中可检测出虫体DNA ,而脾脏中只有对照组可检出虫体DNA。结论　小鼠腹水的PCR检测结果与存活率和腹水虫体镜检结果一致 ,并弥补了镜下检查由主观造成的不足 ;肝、脾、脑等脏器的PCR检测也取得与存活率基本一致的结果 ;总之 ,本研究中的PCR检测结果较客观地反映药物对小鼠的疗效情况 ,为动物实验性弓形虫病的药物疗效考核提供了一个较为简便、灵敏、特异的方法更多还原

【Abstract】 Aim The efficacy of dihydroartemisinine on murine model of acute toxoplasmosis was evaluated by using PCR techniques in our experiment Methods Eight hours after they were challenged intraperitoneally with 2×10 3 RH strain tachyzoites of T gondii,mice were treated with dihydroartemisinine alone(75mg/kg d)or combined with sulfadiazine(100mg/kg d)for 15 days and every 12 hours the drugs were fed the mice Then mice were killed at 9,15,31 days after infection,the peritoneal fluid,livers,spleens and brains were taken out for PCR analysis of DNA of T gondii;brain was taken out from the survival mouse for detecting DNA of T gondii after the treatment was ended for 5 months Results Dihydroartemisinine in combination with sulfadiazine protected 93% mice against death There was significant difference between the survival rate of combination regimens and that of sulfadiazine alone(P<0 05);so did the survival rate between the combination regimens and dihydroartemisinine alone(P<0 01) The PCR results indicated the DNA of T gondii was detected in peritoneal fluid of mice in all other groups except the dihydroartemisinine combined with sulfadiazine group,and PCR were positive in livers and brains of mice in control group and dihydroartemisnine group,but PCR was positive only in spleen of mouse in control group Conclusion The results of PCR detection accord with the survival rate and observation results of T gondii in peritoneal fluids under microscopy,and this makes compensation for the deficiency of observation with naked eyes;the results of PCR detections in livers,spleens and brains are in accordance with the survival rate;this PCR method basically reflects the effects of these drugs on infected mice objectively,provides a more sensitive,specific and convenient method for assessing the effects of drug on experimental toxoplamosis更多还原