【摘要】 目的 :制备用于检测人胚胎组织Tra2α蛋白的特异抗体。方法 :选择Tra2α中特有的一段编码序列 (第 5 2～ 16 5位核苷酸 ) ,通过克隆至pGEX 3x表达载体形成GST融合基因 ,并以诱导表达和纯化的该融合蛋白为抗原免疫新西兰兔 ,获得了抗Tra2α的血清。结果 :免疫印迹 (Westernblot)检测结果显示 ,该血清能特异地与人胎脑组织中的Tra2α蛋白结合。免疫组织化学结果显示 ,该血清能用于人胎脑组织中Tra2α蛋白分布的检测。结论 :所制备的抗血清具有很好的特异性 ,可用于人胚胎组织中Tra2α蛋白的检测。更多还原

【Abstract】 Objective:To prepare specific antibody against the pre mRNA splicing protein Tra2α in human fetal tissues.Methods:A specific antigen fragment of human Tra2α was cloned into the pGEX 3x expression vector to express a recombinant GST Tra2α fusion protein.The fusion protein was expressed,purified and used as an antigen to immunize the New Zealand rabbit,and the polyclonal antibody to Tra2α was obtained.Results:The antibody was successfully used to detect Tra2α protein in human fetal cerebrum with Western blot and immunohistochemistry analysis.Conclusion:This prepard antibody has good specificity and can be used to detect the Tra2α protein in the human fetal tissues.更多还原