【摘要】 目的 :探讨血管内皮细胞生长因子 (VEGF)及其受体在乳腺癌恶性进展中的作用及机制。方法 :使用反转录聚合酶链反应 (RT- PCR)及计算机条带分析半定量方法检测 VEGF4种异构体 (12 1,16 5 ,189,2 0 6 )及 VEGF受体 (flt- 1及 KDR) m RNA在 2 9例乳腺癌组织及相应癌周组织的表达水平。结果 :VEGF的 2种主要异构体 (VEGF12 1,VEGF16 5 )及 KDR在癌组织的表达水平明显高于癌周组织 (P<0 .0 0 1)。flt- 1的表达在癌组织及癌周组织之间则没有显著性差异 (P>0 .5 )。在癌组织及癌周组织 ,VEGF12 1表达和 KDR表达之间具有明显直线正相关及回归依存关系 (P<0 .0 0 1,P<0 .0 2 ) ,而 flt- 1表达水平则没有直线相关关系 (P>0 .1)。乳腺癌组织 VEGF12 1表达量在未绝经组患者高于绝经组 (P<0 .0 1)。结论 :KDR是乳腺癌组织中 VEGF发挥功能的主要受体 ,其表达增高可能是因为受到 VEGF的诱导上调作用 ;体内卵巢激素水平可以影响乳腺癌组织 VEGF表达。更多还原

【Abstract】 Objective:To clarify the role of VEGF and its receptors in breast tumor development.Methods:The mRNA expression of the four alternatively spliced VEGF isoforms (121,165,189 and 206 amino acids) and VEGF receptors (flt 1 and KDR) were examined by semiquantitative reverse transcriptase polymerase chain reaction analysis in breast cancer (29 specimens) and the corresponding adjacent non neoplastic breast tissues (29 specimens).Results:Three VEGF transcripts were detected in both malignant and non malignant breast tissues (121,165,189).The KDR transcripts and the two major VEGF transcripts (VEGF121 and VEGF165) were more abundant in breast carcinomas than in the adjacent non neoplastic breast tissues ( P <0.001). In contrast,the level of expression for flt 1 mRNA was not different in the malignant and the adjacent non neoplastic breast tissues ( P >0.5). Positive linear correlation was observed between VEGF and KDR in the breast carcinomas and adjacent non neoplastic breast tissues ( P <0.001, P <0.02). There was no linear correlation between VEGF and flt 1 expression ( P >0.1). VEGF121 mRNA expression level was significantly higher in premenopausal compared to post menopausal malignant breast tissues ( P <0.01).Conclusion:The intense expression of VEGF, KDR mRNA by breast carcinoma provides strong evidence linking VEGF expression to the angiogenesis associated with breast carcinoma. The positive linear correlation between VEGF expression and KDR expression clearly demonstrates VEGF induced up regulation of VEGF receptor. KDR is the main signaling receptor for VEGF in breast carcinoma, the VEGF/KDR system plays a functional role in the progression of breast carcinoma. Hormonal status can influence VEGF expression level in malignant breast tissues.更多还原