【摘要】 目的 :观察“参臼胶囊”(SJ)对人肝癌细胞 SMMC- 772 1细胞的抑制作用。方法 :采用MTT、软琼脂克隆形成、电镜观察等方法 ,观察 SJ对肝癌 SMMC- 772 1细胞的作用 ,采用免疫组织化学法测定 p5 3、Topo 及 bcl- 2表达。结果 :MTT法测定结果表明 ,SJ对 SMMC- 772 1细胞生长有明显的抑制和杀伤作用 ,且随着 SJ剂量的增高 ,其抑制程度增强 ,两者呈正性相关关系 (r=0 .976 ,P<0 .0 1) ;统计学结果显示 :IC50 ( SJ) <IC50 ( VP1 6 ) ) ,(t=- 10 .84 ,P<0 .0 1) ,表明 SJ对 SMMC- 772 1的抑制作用明显优于 VP1 6 ;软琼脂克隆形成实验结果显示 :SJ可明显抑制 SMMC- 772 1克隆形成 ,表明其具有较强的抑制与杀伤肿瘤干细胞的能力 ,且随剂量增加 ,抑制作用加强 ,呈现良好的量效关系 (χ2 =4 8.6 9,P<0 .0 1;r=0 .5 ,P<0 .0 1) ;在药物延时效应实验中观察到 :SJ对 SMMC- 772 1作用 8h,即已具备延持效应 ,作用 12 h延持效应明显 ,撤药后细胞仍趋向死亡 ,表明 SJ对肿瘤细胞的抑制作用是不可逆转的 ;SJ可抑制肿瘤细胞中 p5 3、Topo 及 bcl- 2的表达。结论 :肝癌细胞 SMMC- 772 1对 SJ较VP1 6 更为敏感 ,提示 SJ可能作为一种较为理想的抗肝癌化疗药物 ;药物延时效应结果为 SJ的临床应用提供了一个有益的启示 :即可在肿瘤?更多还原

【Abstract】 Objective:To study the inhibitory effect of Senjiu (SJ) Capsule on human liver cancer cell line SMMC 7721.Methods:The inhibitory effect of SJ was observed by MTT colorimetric assay, electron microscopy(TEM) and immunohistochemical techniques.Results:SJ significantly inhibited SMMC 7721 cells growth and this inhibitory effect was positively related to the concentration and exposuring time to SJ. Liver cancer cell line SMMC 7721 was more sensitive to SJ than to VP 16.SJ can be used as a new anti tumor drug for liver cancer therapy. While inducing apoptosis, SJ could decrease the expression of Topo Ⅱ and apoptosis associated genes Bcl 2 and p53.Conclusion:Liver cancer cell line SMMC 7721 was more sensitive to SJ than to VP 16.SJ can be used as a new anti tumor drug for liver cancer therapy. The apoptosis of cancer cells induced by SJ is associated with downregulation of protein expression of TopoⅡ,Bcl 2 and p53 genes.更多还原