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中枢神经细胞培养中电刺激的实验研究
Experimental observation of electrical stimulation on cultured nervous system cells
【摘要】 目的了解电针刺激对神经细胞的影响。方法 1日龄Wistar大鼠脑神经细胞体外原代培养 4天 ,第 5日起分为 4组 :第 1组为空白对照组 ,第 2组为放置电极对照组 ,第 3组为低电量刺激组 (脉冲幅度 :连续波形 80mA ,频率 2 0Hz) ,第 4组为中电量刺激组 (脉冲幅度 :连续波形 12 0mA ,频率 2 0Hz)。每天电刺激 2次 ,每次 3 0分钟 ,共 7天。第 10天开始显微镜下观察其与对照组在神经细胞形态上的变化。结果电刺激可以使神经细胞的突起有趋向性延长和连接。结论在神经细胞的培养过程中 ,电刺激对神经细胞生长具有一定影响。
【Abstract】 ObjectiveTo explore the influence of electrical stimulation on nervous cells.MethodsOn 5th day the brain primitive nervous cells of rat brain, in vitro culturing, were divided into 4 groups, in order of control group, electrical pole control group, low stimulation group, and high stimulation group. The cells were stimulated with electro-acupuncture apparatus for 7 days, 30 minutes once, twice a day. On 10th day, the cells were observed microscopically for 3 days and taken photos.ResultsElectrical stimulation is helpful to raise the survival rate of cell.Conclusions Electrical stimulation influences morphological change of nervous cells during culturing.
- 【文献出处】 中国康复理论与实践 ,Chinese Journal of Rehabilitation Theory & Practice , 编辑部邮箱 ,2002年11期
- 【分类号】R329
- 【被引频次】19
- 【下载频次】298