【摘要】 目的　调查戊型肝炎病毒 (HEV)Ⅳ型在北京地区的急性散发性肝炎中的分布。方法采用RT nPCR的方法检测 30 0例急性散发性肝炎患者中HEVRNA ,并对阳性产物进行克隆测序 ,然后对其基因型进行分析。结果　在 30 0例急性散发性肝炎患者中PCR阳性为 2 5例 ,测序证实 2 5例PCR阳性者中 ,2 4例为HEV的基因序列 ;其中 9例为HEVⅠ型 ,15例为HEVⅣ型 ,占HEV感染的 6 2 .5 %(15 / 14 )。结论　在北京地区的部分急性散发性肝炎中HEVⅣ型感染占戊型肝炎的较大比例。更多还原

【Abstract】 Objective To investigate the distribution of HEV genotype Ⅳ among the patients with sporadic acute hepatitis in Beijing. Methods HEV RNA were detected with RT nPCR and the positive PCR products were cloned and sequenced. The genotype of the isolated sequences was analyzed with the molecular biological software. Results 25 of 300 sera from patients with acute hepatitis were positive for RT nPCR, 24 of 25 PCR positive products were confirmed to be related to HEV sequence by sequencing. 9 of 24 sequences belong to HEV genotype Ⅰ and 15 of 24 sequences belong to HEV genotype Ⅳ. Conclusion The infection rate of HEV genotype Ⅳ is mores preading than that of HEV genotype Ⅰ among the patients with the diagnosis of sporadic acute hepatitis in Beijing. [更多还原