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戊型肝炎病毒Ⅳ型在300例急性肝炎中的感染比例

The distribution of HEV genotype Ⅳ among 300 cases of patients with sporadic acute hepatitis in Beijing

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【作者】 蓝海云王佑春李卓张华远郝娃辜文洁林京香李河民Tim J Harrison

【Author】 LAN Haiyun *, WANG Youchun, LI Zhou, ZHANG Huayuan, HAO Wa, GU Wenjie, LIN Jingxiang, LI Hemin, Tim J Harrison. *Department of Hepatitis, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China

【机构】 中国药品生物制品检定所北京市佑安医院英国伦敦大学皇家免费医学院

【摘要】 目的 调查戊型肝炎病毒 (HEV)Ⅳ型在北京地区的急性散发性肝炎中的分布。方法采用RT nPCR的方法检测 30 0例急性散发性肝炎患者中HEVRNA ,并对阳性产物进行克隆测序 ,然后对其基因型进行分析。结果 在 30 0例急性散发性肝炎患者中PCR阳性为 2 5例 ,测序证实 2 5例PCR阳性者中 ,2 4例为HEV的基因序列 ;其中 9例为HEVⅠ型 ,15例为HEVⅣ型 ,占HEV感染的 6 2 .5 %(15 / 14 )。结论 在北京地区的部分急性散发性肝炎中HEVⅣ型感染占戊型肝炎的较大比例。

【Abstract】 Objective To investigate the distribution of HEV genotype Ⅳ among the patients with sporadic acute hepatitis in Beijing. Methods HEV RNA were detected with RT nPCR and the positive PCR products were cloned and sequenced. The genotype of the isolated sequences was analyzed with the molecular biological software. Results 25 of 300 sera from patients with acute hepatitis were positive for RT nPCR, 24 of 25 PCR positive products were confirmed to be related to HEV sequence by sequencing. 9 of 24 sequences belong to HEV genotype Ⅰ and 15 of 24 sequences belong to HEV genotype Ⅳ. Conclusion The infection rate of HEV genotype Ⅳ is mores preading than that of HEV genotype Ⅰ among the patients with the diagnosis of sporadic acute hepatitis in Beijing. [

【基金】 英国WellcomeTrust基金资助课题
  • 【文献出处】 中华微生物学和免疫学杂志 ,Chinese Journal of Microbiology and Immunology , 编辑部邮箱 ,2002年03期
  • 【分类号】R512.6
  • 【被引频次】28
  • 【下载频次】83
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