【摘要】 目的　了解肥胖型 2型糖尿病 OL ETF鼠胰岛β细胞是否存在凋亡及β细胞凋亡与糖尿病发生发展的关系。　方法　 OL ETF及对照鼠 L ETO各 2 0只 ,胰腺切片用 HE染色和 TU NEL方法观察胰岛β细胞凋亡的情况。　结果　 (1) OL ETF鼠糖尿病发病率为 95 % ,L ETO未发生糖尿病。 (2 )仅在 OL ETF鼠胰腺切片中观察到凋亡的β细胞数目增高的现象。 (3)各周龄 OL ETF鼠胰岛β细胞凋亡的百分数明显高于 L ETO鼠 (P=1.2 6× 10 - 1 7)。 (4) OL ETF鼠胰岛β细胞凋亡的百分数在糖尿病发病后 4周较糖尿病发病前增高。　结论　 OL ETF鼠胰岛β细胞在糖尿病发病前存在高水平的凋亡 ,糖尿病发病后进一步增高更多还原

【Abstract】 Objective To determine whether the β cells apoptosis exits in the obesity associated type 2 diabetic rats OLETF and the relationship between the apoptosis and development of type 2 diabetes. Methods Twenty OLETFs and twenty LETOs as control were used. HE staining and TUNE were the way to examine apoptotic β cell in pancreatic sections. Results (1) The incidence of diabetes in OLETF rats was 95%. None of diabetic rat was found among LETO rats. (2) Apoptotic cells only appeared in pancreatic sections from OLETF rats. (3) The percentage of islet β cell apoptosis in OLETF rats was higher than that in LETO rats (P=1.26×10 -17 ). (4) In OLETF rats, the percentage of islet β cells apoptosis was higher in the ages of 4 weeks after the onset of diabetes than that in the ages of four weeks before the onset of diabetes. Conclusion There was a high level of β cell apoptosis in OLETF rats prior to the onset of diabetes and a further increase of apoptosis after diabetes.更多还原