【摘要】 目的:从细胞及分子水平分析低剂量米非司酮在子宫内膜对胚泡接受性中的作用.方法:以组织学方法观察内膜形态变化;免疫组织学方法测定LIF及IL-6在子宫内膜的基因表达以及用半定量反转录—聚合酶链反应（RT-PCR）测定内膜雌、孕激素受体nRNA水平等方法来评估子宫内膜的成熟度.结果:米非司酮在剂量为0.03mg·kg^-1·d^-1时,可明显延缓子宫内膜的成熟,抑制间质细胞蜕膜化,降底LIF和IL-6在内膜的基因表达和增高内膜雌、孕激素受体mRNA水平.结论:小剂量米非司酮可直接作用于子宫内膜,影响内膜的成熟而抑制内膜对胚泡的接受性.更多还原

【Abstract】 To determine the effect of a low-dose mifepristone regimen on endometrial receptivity in guinea pigs. METHODS: Histologic evaluation for endometrial staging and immunoreactivity of cytokines [ leukemia inhibitory factor (LIF) and interleukin-6 (IL-6)] were preformed on fixed endometrial tissue, and relative abundance of endometrial estrogen (E), and progesterone (P) receptor mRNA were evaluated with semiqantitative reverse transcriptase polymerase chain reaction (RT-PCR) in which cyclophilin mRNA, a housekeeping gene product, was co-amplified as the reference standard. RESULTS: Mifepristone at 0.03 mg·kg-1·d-1 induced a delay in endometrial maturation. The staining intensity of LIF and IL-6 decreased in treated guinea pigs. E and P receptor mRNA increased significantly and uterine stage delayed in treated guinea pigs. CONCLUSION: Mifepristone at 0.03 mg·kg-1·d -1 delayed development of endometrium. This study provides further evidence that endometrial maturation can be altered without affecting ovarian ovulatory function.更多还原