【摘要】 利用草鱼原代肝细胞培养技术评价二口恶 口英 毒性效应,测定结果与离体培养生物测试方法、高分辨质谱分析方法的测定结果进行了比较.研究结果表明,同步获得的2,3,7,8-四氯代二苯并二口恶 口英 (2,3,7,8-TCDD)对草鱼原代肝细胞和离体H4IIE细胞的EROD酶活力诱导的剂量-效应关系标准曲线,前者的EC50值比后者的EC50值高出约10倍,草鱼原代肝细胞对2,3,7,8-TCDD的灵敏度比离体H4IIE细胞的要低,2,3,7,8-TCDD对这两种细胞生物毒性效应存在着差异.测定同一环境样品中的二口恶 口英 类化合物毒性时,离体培养方法的测定结果比原代培养方法的测定结果更接近化学分析结果,但原代培养比离体培养更能如实地反映环境中污染物的生物毒性和毒理动力学过程.更多还原

【Abstract】 Toxic effects of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) were evaluated using grass carp primary hepatocyte culture technique; and the defermination results were compared with those from in vitro culture bioassay and HRGC/HRMS-MID analysis. The study results demonstrated the standard curve of the 2,3,7,8-TCDD and the dose-response relationship induced by the EROD ferment activity of grass carp primary hepatocyte and in vitro H4IIE cell. The in vitro H4IIE cell was more sensitive to 2,3,7,8-TCDD than the grass carp primary hepatocyte; and its EC50 value was lower about 10 times than that of the grass carp primary hepatocyte. The toxic effects of the 2,3,7,8-TCDD on these two kinds of cells were different. When the toxic effects of PCDD/F in the same environmental samples were determined, the results of in vitro culture technique were more closed to the results of chemical analysis than those of primary culture technique; but the primary culture can more realisticly reflect the biotransformation process and the biotoxicity of pollutants in environment.更多还原