【摘要】 研究了2,4-二氯苯胺、3,4-二氯苯胺、2,5-二氯苯胺、2,3-二氯苯胺对斑马鱼的急性毒性,96 h LC50分别为7.79、6.08、5.23、0.49 mg/L.为阐明二氯苯胺类化合物对水生生物抗氧化酶的早期影响,将鲫鱼暴露于不同浓度的4种化合物中,研究鲫鱼血清SOD和GSH-PX活性短期(48h)内的变化,结果表明,在实验设置浓度下,随着暴露浓度升高,与空白对照组相比,4种化合物对SOD活性抑制作用明显,对GSH-PX活性先激活后抑制,表明4种化合物对鲫鱼血清SOD和GSH-PX活性有显著影响,与4种化合物96h LC50相比,引起生化效应的暴露浓度明显降低、且反应快速,SOD和GSH-PX相结合作为该类化合物对水环境污染胁迫的敏感生物指示物具有一定的可行性.更多还原

【Abstract】 The acute toxicity of 2,4-dichloroaniline, 3,4-dichloroaniline, 2,5-dichloroaniline and 2,3-dichloroaniline to zebra fish were studied, and the 96 h LC50 were 7.79, 6.08, 5.23 and 0.49 mg/L respectively. In order to explain the early influences of dichloroanilinated compounds on antioxidant enzymes of aquatic organisms, Carassius auratus was exposed to different concentrations of these four compounds to study the change of SOD and GSH-PX activities of Carassius auratus serum in short term (48h). The results showed that under the concentrations of this experiment, with the exposing concentrations increasing, SOD activities were obviously restrained and GSH-PX activities were activated previously and then restrained as compared with the control groups; and that these compounds had significant influence on SOD and GSH-PX activities; comparing with 96 h LC50 of these compounds, exposing concentrations which induced bio-chemical effect were decreased markedly; and the response was speedy; and it was feasible definitely for SOD combined with GSH-PX to become biomarkers of these chemicals?polluting menace on water environment.更多还原