【摘要】 目的　研究灰旱獭感染乙型肝炎病毒 (HBV)后的血清学反应。方法　实验前普查 2 8只实验灰旱獭对乙肝病毒标志物 (HBVM)的血清学变化 ,选阴性健康旱獭用含HBVDNA的人血清进行皮下、肌内和腹腔多点注射 (4 96× 10 6cop ml× 3ml) ,接种后 1～ 8周 (每日 )和 2～ 12个月 (每 1～ 2个月 )观察行为变化 ,检测HBVM ,HBVDNA和ALT AST。结果　攻毒前抗 HBc+率为 7 1% (2 2 8) ,攻毒后抗 HBc+率为 31 8% ,HBsAg+率为 4 3% ,HBVDNA+率为 16 6 % ,ALT一周后开始升高 ,2～ 3个月达高峰 (高于正常 2～ 9倍 )并持续 6个月 ,AST低平曲线高峰迟发一个月。全程死亡 7只 (2 5 % ) ,攻毒后死亡 1只 (1 7)。结论　攻毒后HBV和ALT AST的变化提示HBV感染后能产生肝细胞炎症反应更多还原

【Abstract】 Objective To study serum tests on HBV infection of grey marmota(Marmota baibacina).Methods 28 MHV-negative and healthy marmots were injected with HBV-DNA of 4.96×10 6 copise/ml×3ml in the routes of subcutaneous, muscle and abdominal cavity, respectively. HBV-M, HBV-DNA and ALT/AST were observed every week from the first to eighth week and every month from the third to 12th month after injection. Results The positive rate of 7.1%(2/28) of HBcAb was before injection, while HBcAb, HBsAg and HBV-DNA were 31.8%, 4.3% and 16.6% respectively after challenge. ALT started to rise one week later; the peaks were within 2-3 months, being 2 to 9 times higher than the control group, then reduced gradually to the normal after 6 months of injection. The peak of AST was one month later than ALT. Seven bodies died in the test period(25%), and one died right after injection(1/7). Conclusion The above changes of HBV and ALT/AST indicated that there was inflammational response in the liver cells of marmots injected with HBV.更多还原