【摘要】 目的　解决南方红豆杉生长缓慢的问题。方法　考察红豆杉细胞悬浮培养过程中磷的消耗 ,并研究了碳、氮、磷对细胞生长及细胞活性的影响。结果　碳源在悬浮培养中期已经消耗殆尽 ,后期碳源的缺乏限制了细胞的生长。结论　补加碳源组与对照组相比 ,细胞生长率和细胞密度明显提高 ,其中细胞生长率约提高了 83%。更多还原

【Abstract】 Object To solve the problem that Taxus chinensis var. mairei (Lemee et Lévl.) Cheng et L. K.Fu grows slowly. Methods The consumption of phosphorus during the cell suspension culture and the effect of fed-batch carbohydrate, nitrogen and phosphorus on cell growth and living-cell activity were assayed. Results The carbohydrate was exhausted in the middle phase of suspension culture, dificiency of carbohydrate led to inhibition of cell growth in the late culture. Conclusion Compared with the control group, the cell growth rate and the cell density in the fed-batch carbohydrate group were increased significantly, and the cell growth rate was up to 83%.更多还原