【摘要】 在云南、广西大面积的三七栽培过程中三七常因镰刀菌侵染造成三七根腐病。 2 0 0 1年在分析比较健康及感染三七根腐病三七根的蛋白质图谱的基础上 ,为进一步研究三七抗根腐病品种的分子标记 ,本实验用健康及感染三七根腐病三七根样品的全蛋白质提取液进行了蛋白免疫印迹分析。单克隆抗体(Mab7)是由一种小鼠杂交细胞系 ( #4 90 ;ATTCHB - 1 2 0 2 9)针对Cronartiumribicola担孢子蛋白产生的抗体。它被鉴定为一种免疫蛋白I,能疹断不同地理范围内疱锈菌样品中 70道尔顿热休克蛋白抗原 (CrorⅡ )。它被用于针叶树病原系统蛋白组学研究。本实验用单克隆抗体 (Mab7)用分析了三七健康 (主根、须根 )及感病组织的蛋白免疫印迹。并用PDIquantityone软件包详细分析了其蛋白印迹。Mab7在健康组织中在分子质量 1 3.2～ 5 2 .3u之间显示出 9条不同的免疫印迹谱带 ,其中分子质量 2 5 .5及 2 6.4u位置处出现两条比较强的谱带 ,在须根组织中只有在 1 4.4道尔顿位置处出现一条谱带。在而在感病组织中显示更少的谱带 ,而且带型截然不同。用单克隆抗体 (Mab7)监测组织中带型的变化有助于育种中抗病性的研究。进一步研究带型的变化与病害严重度之间的关系正在进行中。结果表明单克隆抗体Mab7作为免疫疹断工具可用于三七品种?更多还原

【Abstract】 During large scale domestic cultivation in Yunnan and Guangxi Province, China, pseudoginseng ( Panax notoginseng ), is often attacked by Fusarium solani , causing pseudoginseng root rot disease. Recently, the protein profiles of healthy roots and disease infected roots were generated and compared. As a further step towards generating molecular markers for the development of root rot disease resistant pseudoginseng cultivars, Western immnunoblot analyses were conducted with the total protein extracts from infected and healthy pseudoginseng root samples. A monoclonal antibody (Mab7) developed during conifer pathosystem proteomics studies was used in this Western blot study. Mab 7 detected up to nine bands on the Western blot in healthy root sample. Fewer bands and a different banding pattern were detected in infected roots. Our results indicated that this monoclonal antibody (Mab7) could be used in the future for pseudoginseng cultivar analyses and disease defense response analyses.更多还原