【摘要】 利用改进的聚丙烯酰胺凝胶电泳 (PAGE) ,对玉米种子清蛋白和球蛋白进行研究。结果用含有NaCl的球蛋白提取液 Ⅰ 提取 5次蛋白质后 ,清蛋白和球蛋白几乎全部被提取 ;而用不含NaCl的清蛋白提取液 Ⅱ 提取 4次蛋白质后 ,再用提取液 Ⅰ 提取 ,可得到球蛋白组分 ,而且在清蛋白中也有相同迁移位置的谱带存在。表明对球蛋白进行提取操作时 ,总有清蛋白相伴随 ,而对清蛋白进行提取分析时一般不会有球蛋白的存在 ;球蛋白的遗传组分较清蛋白少 ,并在清蛋白中一般都有相同分子量的组分存在更多还原

【Abstract】 Compare analysis of maize seed albumin and globulin were made by improving PAGE Almost all of the albumin and globulin were extracted after using extract solution Ⅰ which included NaCl five times But only the globulin were obtained by using extract solution Ⅱ which didn’t include NaCl four times and extract solution Ⅰ one time The globulin obtained had bands which had the same mobility as the albumin The result showed that the albumin had also been extracted while we extracted globulin, but the globulin hadn’t been extracted while we extracted albumin The gentic elements of globulin, which had the same molecular weight as albumin, were less than albumin’s更多还原