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吲哚美辛对体外培养的RPE细胞增殖及DNA合成的影响(英文)

Inhibition of indomethacin on proliferation and DNA synthesis of cultured hRPE cells

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【作者】 李文生； 温俊； 姜德咏； 丁建光； 吴荣瀚；

【Author】 Li Wensheng,Wen Jun , Jiang Deyong ,Dmg Jianguang ,Wu RonghanDepartment of Ophthalmology, Second Affiliated Hospital of Kunming Medical College, Kunming 650101 ; Department of Pharmacology, Kunming Medical College, Kunming 650031 ;Department of Ophthalmology, Second Affiliated Hospital of Xiangya Medical College, Central South University, Changsha 410011

【机构】 昆明医学院第二附属医院眼科； 昆明医学院药理教研室； 中南大学湘雅二院眼科； 中南大学湘雅二院眼科 650001；

【摘要】 目的探讨吲哚美辛(IN)对体外培养的人胚胎视网膜色素上皮(RPE)细胞的抑制作用及DNA合成的影响。方法 分别用核酸蛋白分析仪测定加入50、100、200、400、600μmol/L IN作用24 h后RPE细胞DNA质量浓度的变化和MTT法测定加入100、200、400、600、800、1 000 μmol/L IN 12 h后RPE细胞数量的改变。 结果 前者其细胞DNA质量浓度(单位:μg/ml)分别为101.171 2±15.512 4、88.640 0±13.584 5、72.365 1±7.796 9、5.908 9±10.722 9、51.223 6±8.775 7。与对照组21 3.735 1±83.157 2相比,差异有显著性(q值分别为5.481、6.091、6.883、7.490、7.912,P值分别为0.000、0.000、0.000、0.000、0.000);后者其细胞A值分别为0.236 7±0.054 6、0.168 7±0.069 5、0.081 9±0.034 6、0.065 6±0.017 6、0.055 4±0.028 6、0.050 8±0.027 7。与对照组0.267 4 ±0.043 0相比,差异有显著性(q值分别为1.442、4.621、8.682、9.447、9.925、10.140,P值分别为0.158、0.000、0.000、0.000、0.000、0.000)。 结论 IN可抑制体外培养的RPE细胞的DNA合成及增殖。

【Abstract】 Objective To investigate the inhibitory effects of indomethacin (IN) on proliferation and DNA synthesis of cultured human fetal retinal pigment epithelium ( hRPE) cells in vitro. Methods Primary culture and subculture of hRPE cells were established in vitro first. Cultured hRPE cells were treated by various concentrations 50,100,200,400,600μ mol/L(final concentration ) of IN for 24 h. After 24 h, the amount of DNA in RPE cells was determined by the absorbance at 280 nm of Nucleic Acid 8 Protein Analysis. Cells proliferation of RPE were measured with methyl thiazolyl tetrazolium ( MTT) assay method by adding 100,200,400,600,800,1000 μ mol/L(final concentration) of IN for 12h. Results After added various concentrations of IN,the DNA concentrations were ( 101. 171 2 ±15. 512 4 ) , ( 88. 640 0 ± 13. 584 5 ) , ( 72. 365 1 ±7. 796 9 ) , ( 59. 908 9±10. 7229) ,(51. 223 6±8. 775 7)μ g/ml respectively ,P values were 0. 000 ,0. 000 ,0. 000 ,0. 000,0. 000(q-test) as compared to that (213.735 1 ± 83. 1572)μ g/ml in 0 μg/L IN. The A values added 100,200,400,600,800,1000μmol/L of IN were (0.2367±0.0546) ,(0. 1687 ±0.0695) ,(0.081 9 ±0.03461) ,(0.0656±0.0175 9) ,(0.0554±0.02865) , (0. 0508 ±0.027 75) respectively,P values were 0. 158 ,0. 000 ,0. 000,0. 000,0. 000 ,0. 000( q-test) as compared to (0.2674±0.04302) of A value of 0 μg/L IN. Conclusion The data suggested that IN can inhibit DNA synthesis and proliferation of hRPE cells in vitro in a dose-dependent manner.

【关键词】 retinal pigment epithelium cell indomethacin proliferation DNA synthesis；
【Key words】 retinal pigment epithelium cell indomethacin proliferation DNA synthesis；
  • 【文献出处】 眼科研究 ,Chinese Ophthalmic Research , 编辑部邮箱 ,2002年06期
  • 【分类号】R774.1
  • 【下载频次】36
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