【摘要】 目的　研究纤维蛋白溶解酶 (简称纤溶酶 )联合透明质酸酶诱发玻璃体后脱离 (PVD)。方法　 16只兔随机分为A、B、C三组各 8、4、4只 ,均右眼为实验眼 ,左眼对照。A组实验眼玻璃体腔内注射纤溶酶 1U和透明质酸酶 2 0U ,B组纤溶酶 1U ,C组透明质酸酶 2 0U ,对照眼均注射BSS液。术后 7天内行B超、ERG、扫描及透射电镜等检查。结果　扫描电镜确诊A组实验眼完全性PVD 10 0 %,B组实验眼部分性PVD 75 %,C组实验眼及对照眼无PVD。实验及对照眼ERG振幅无明显下降 (P >0 0 5 ) ,透射电镜等检查无眼内毒性。结论　纤溶酶 1U联合透明质酸酶 2 0U兔玻璃体腔内注射能诱导出完全性PVD ,且无眼内毒性。更多还原

【Abstract】 ObjectiveTo investigate whether an injection of plasmin combined with hyaluronidase can induce posterior vitreous detachment(PVD)and the safety of these two proteases.Method16 rabbits were assigned to three groups(8 in group A,4 in B,4 in C).All the right eyes were injected proteases.Group A received a vitreous injection of plasmin 1 U combined with hyaluronidase 20 U(0.1 ml).Group B received plasmin alone.Group C received hyaluronidase alone.16 left eyes were injected 0.1 ml BSS as controls.All the rabbits were monitored by clinical electroretinography(ERG)and B-ultrasonography and sacrificed 7 days later.Eyes enucleated were examined by scanning and transmission electron microscopy.ResultsIn group A,the inner limiting membrane (ILM) was smooth expect the vitreous base,indicating complete PVD(100%).In group B,indicated partial PVD(75%).In group C and all the control eyes,no PVD.The b-wave and a-wave amplitudes of ERG showed no evident difference compared with preinjection values in all groups(P>0.05).Transmission electron microscopy confirmed the retina was not damaged.ConclusionVitreous injection of plasmin 1 U combined with hyaluronidase 20 U can induce complete PVD without intraocular toxicity at 1 week after injection.However,plasmin 1 U alone can only induce partial PVD and hyaluronidase 20 U alone is not effective.更多还原