【摘要】 小麦品种苏麦 3号高抗由禾谷镰刀菌 (FusariumgraminearumSchw .)引起的小麦赤霉病 ,已成为全世界广泛应用的抗源。为更精确地研究苏麦 3号的赤霉病抗性 ,实验用感病品种川 980为供体亲本 ,苏麦 3号为轮回亲本 ,通过不断回交和自交 ,把川 980的赤霉病感病基因导入苏麦 3号 ,创造了感赤霉病的苏麦 3号近等基因系 (S0 16 ) ,并得到以下结果 :(1)育成的苏麦 3号感赤霉病近等基因系 ,经两年多点抗赤霉病性状鉴定 ,表明该近等基因系感赤霉病感病性稳定 ,而其他形态特征、特性与苏麦 3号一致。 (2 )用RAPD、RFLP分析抗、感苏麦 3号近等基因系之间的差异 ,RFLP分析发现该近等基因系在 2D染色体上存在差异。RAPD的随机扩增产物OPH1914 0 0 可能与近等基因系中的赤霉病感病基因存在连锁。 (3)用近等基因系验证已报道的与小麦抗赤霉病基因有关的分子标记 ,均未发现这些分子标记与导致该近等基因系抗性差异的基因有关。更多还原

【Abstract】 Wheat scab can cause significant yield lost and quality decrease as well as toxicoses in animals and humans. Sumai 3, a resistant cultivatr to scab, is widely used in the wheat breeding of scab resistance. To study the inheritance of resistance to scab in Sumai 3, the highly susceptible cultivar Chuan 980 was crossed with Sumai 3 and backcrossed with Sumai 3 as a recurrent parent for seven times, thus a near isogenic line S016 susceptible to scab was developed. S016 was evaluated in five regions and a period of two years for resistance to spread of scab in spike. Results showed that S016 was as highly susceptible to wheat scab as one of the parent Chuan 980. Molecular markers (RFLP, RAPD) were screened to identify chromosome regions of negative element of resistance gene (s) in S016. The DNA polymorphism between the near isogenic lines was showed using six restrict enzymes ( Eco RⅠ, Eco RⅤ, Hin dⅢ, Dra Ⅰ, Bam HⅠ, Xba Ⅰ) and 85 probes located in wheat chromosome 2D. Among the amplified bands of 450 10 mer random primers, OPH19 1400 and OPH19 1200 were perhaps linked to negative regulator element. Some reported probes linking to scab resistance gene(s) did not show any polymorphism between this near isogenic line and their generation.更多还原