【摘要】 在酸性条件和阳离子表面活性剂CTMAB存在下 ,染料孔雀石绿与DNA发生作用 ,在 30 0 0nm ,337 6nm以及 4 5 0 0～ 5 0 0 0nm范围内产生强烈的共振散射峰 .根据 337 6nm处的共振散射信号 ,可以测定 0～ 2 0 μg/mL的小牛胸腺DNA和鱼精子DNA .检测限分别达 6 4 3ng/mL和 4 4 7ng/mL ,4个合成样的分析结果令人满意更多还原

【Abstract】 In acid medium, dye malachite green (MG) and cationic surfactant cetyl trimethyl ammonium bromide (CTMAB) can react with Deoxyribonucleic acids (DNAs), resulting enhanced resonance light scattering (RLS) signals at the wavelength of 300 0 nm, 337 6 nm, and the range between 450 0-500 0 nm. By using the enhanced RLS intensity at 337 6nm, 0 0-2 0 μg/mL ctDNA and fsDNA can be determined with the determination limits of 64 3 ng/mL and 44 7 ng/mL, respectively. The present method was successfully applied to the determination of nucleic acid in synthetic samples.更多还原