【摘要】 目的　研究结膜下注射环孢霉素 A(cyclosporin A,Cs A)微粒体对兔高危眼角膜移植排斥反应的作用。方法　以生物降解医用材料乙交酯 -丙交酯 (polylactic- co- glycolic acid,PL GA )为载体 ,用乳化 -溶剂挥发法制备含 Cs A的 PLG A微粒体 ;观察分别应用 Cs A微粒体或 Cs A溶液角膜移植术后植片存活天数、排斥率及应用不同制剂过程中兔眼的药物反应性。结果　角膜植片平均存活天数及排斥率在 Cs A微粒体组分别为 86.3 0 d± 9.50 d和 2 0 % ,在 Cs A溶液组分别为 71.10 d± 2 5.50 d和 4 0 % ,2组比较植片平均存活天数有显著性差异 (P<0 .1) ,而植片排斥率无显著性差异 (P>0 .1) ;Cs A微粒体对兔眼的药物刺激性小。结论　结膜下注射 Cs A微粒体能够有效抑制角膜移植排斥反应 ,是一种较有前景的缓释制剂。更多还原

【Abstract】 Objective To test efficacy of microspheres of polylactic-co-glycolic acid (PLGA) containing cyclosporin A in the prevention of corneal allograft rejection in the rabbits. Methods Microspheres of PLGA containing cyclosporin A was prepared by a solvent evaporation and solvent extraction process; We have observed survival time and rejection rate of grafts and drug reaction to the rabbits eyes respectively when using microspheres of PLGA and cyclosporin A solution in the prevention of high risk corneal allograft rejection. Results Grafts mean survival time and rejection rates were 71.10d±25.50d and 40% in the group which used microspheres of cyclosporin A and in the other group they were 86.30d±9.50d and 20%. There was significant difference in the mean survival time of grafts between two groups, but there was no significant difference in the grafts rejection rates between two groups. Comparing two formulations, the group of microspheres had little drug reaction to the rabbit eyes.Conclusion The microspheres is promising to be developed as a long-acting cyclosporin A formulation.更多还原