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红莲型水稻不育系和保持系线粒体基因组BAC文库的构建
BAC Library Construction for Mitochondrial Genome of Sterile and Maintainer Lines from HL Rice
【摘要】 以红莲型 (HL )细胞质雄性不育系和保持系为材料 ,构建了水稻线粒体基因组的 BAC文库。每个文库保存约2 30 0个菌落 ,外源插入片段介于 9~ 2 5 kb之间。以线粒体基因为探针对文库进行菌落原位杂交验证 ,均筛选到了阳性克隆。构建的两个文库为进一步研究水稻线粒体基因组的结构特点 ,为克隆与红莲型水稻细胞质雄性不育相关的线粒体基因奠定了基础
【Abstract】 Higher plant mitochondria plays a great role in the process of microspore development, and is closely associated with cytoplasmic male sterility (CMS). The library construction of mitochondrial genome is a necessary step to clone gene and research the structure of the genome. So here, the sterile line and maintainer lines of HL-type rice were used as materials, and BAC libraries of their genome were constructed. Each library consisted of about 2300 clones with insert size ranging from 9 to 25 kb. With mitochondrial genes as probes, positive clones were found. It was calculated that each library contains approximate 40 times of the rice mitochondrial genome.
【Key words】 Cytoplasmic male sterility; Mitochondrial genome; Bacterial Artificial Chromosome (BAC);
- 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,2002年06期
- 【分类号】S511
- 【被引频次】16
- 【下载频次】203