【摘要】 探讨一种新近克隆的特异表达在造血系统组织和细胞中的胚胎发育相关新基因 (EDAG 1)的生物学功能。构建EDAG 1真核表达载体pcDNA3.1(+) EDAG 1,以脂质体法将其稳定转染入NIH3T3细胞中 ,结果发现高表达EDAG 1的NIH3T3细胞失去了正常的平行走向及接触抑制现象 ,侵袭力较正常增强约 10倍并获得锚定不依赖生长能力。将该细胞株皮下接种裸鼠 ,受鼠在 4周左右 10 0 % (6 / 6 )成瘤。这些结果表明EDAG 1是一种具有转化活性的新基因 ,可能与肿瘤发生、发展密切相关更多还原

【Abstract】 To study the biological function of EDAG-1, a novel gene cloned by our lab recently, the EDAG-1 cDNA was subcloned into pcDNA3.1(+) vector and transfected into NIH3T3 cells by Lipofect AMINE methods. Overexpression of EDAG-1 in NIH3T3 cells resulted in loss of cell contact inhibition, anchorage-independent growth in soft agar and tumor formation in nude mice. These results suggest that EDAG-1 is a transforming gene and may be associated with the development of tumors, especially in hematopoietic system.更多还原