【摘要】 目的 :探讨抑制枯否细胞对肝细胞线粒体再灌注损伤的影响。方法 :大鼠分为对照组和实验组 ,第 1、2日经鼠尾静注氯化钆 (7mg/kg) ,对照组注射等量生理盐水 ,第 3日手术使肝缺血 2h后分别复流2h、6h和 18h ,测定肝组织和肝细胞线粒体的丙二醛 (MDA)和血清转氨酶ALT含量 ,观察肝细胞及线粒体超微结构的变化。结果 :实验组复流 2h、6h肝细胞线粒体MDA含量 (nmol/ g)分别为 1.81±0 .2 4和 1.91± 0 .5 6 ,对照组分别为 4 .89± 2 .91(P <0 0 5 )和 2 .93± 0 .5 1(P <0 0 5 ) ,实验组复流 6h肝组织MDA醛含量为 3.5 0± 2 .0 6 ,对照组为 7.6 5± 2 .4 6 (P <0 0 5 )。随着复流时间的延长 ,对照组出现核固缩 ,染色质边集 ,细胞器减少直至肝细胞破坏 ,而实验组在复流 18h后仍可见肝细胞结构基本正常 ,线粒体的损伤程度也明显减轻。结论 :抑制枯否细胞可以减轻肝细胞线粒体的再灌注损伤。更多还原

【Abstract】 Objective:To research the effect on reperfusion injury of hepatocellular mitochondria in rats by inhibition of kupffer cells.Methods:The rats in experimental group were pretreated with Gadolinium(7mg/kg) by introveinal injection for 2 days,and the rats in the control group were treated with saline solution.Two,six and eighteen hours of reperfusion of the liver arter 2 hr of ischemia were performed by operation on the third day.The concentrations of alanine aminotransferase(ALT),of malondialdehyde(MDA)in hepatic tissue and hepatocellular mitochondria were determined.The changes of morphology of hepatic tissue in optical and ultrastructure of hepatocyte and hepatocellular mitochongdria were observed.Results:The contents of MDA of hepatocellular mitochondria in 2 and 6 hours of reperfusion were (1.81±0.24)nmol/g and (1.91±0.56)nmol/g respectively in experimental group,and the contents of MDA of hepatic tissue in 6 hours of reperfusion in experimenta group was (3.50±2.06) nmol/g,and the results in contrast group were (4.89±2.91) nmol/g(P<0 05),(2.93±0.51) nmol/g(P<0 05) and (7.65±2.46) nmol/g (P<0 05) respectively.With the prolongation of the time of reperfusion,karyopyknosis,condensed chromation near nuclear membrane and decreased subcellular structures could be observed,and hepatocyte be demolished when the injury was severe in contrast group,while general hepatocellular appearance was still observed even though in 18 hours of reperfusion in experimental group.And the extent of injury in mitochondria was evidently aba ted.Conclusion:Inhibiting Kupffer’s cells can attenuate the reperfusion injury of hepatocellular mitochondria.更多还原