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杀螨剂微量筛选技术研究

Study on the Method for Acaricidal Microscreening

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【作者】 王松尧; 柴伟纲; 傅荣幸; 朱卫刚; 吴永刚;

【Author】 WANG Song yao *, CHAI Wei gang, FU Rong xing, ZHU Wei gang, WU Yong gang(Zhejiang Branch of National Pesticide R&D South Centre,Hangzhou 310023,China)

【机构】 国家南方农药创制中心浙江基地; 国家南方农药创制中心浙江基地 浙江杭州310023; 浙江杭州310023; 浙江杭州310023;

【摘要】 介绍了一种杀螨剂微量筛选方法。用直经为 9cm、盖子开孔的塑料培养皿 ,中心处放一直径为 2 cm的蚕豆叶片 ,下垫脱脂棉 ,加适量水保湿 ,其上挑接雌成螨 10头 /皿 ,使其产卵、孵化。然后用 Potter喷雾塔对成螨、幼 (若 )螨和卵同时进行定量喷药处理 ,并定期考查结果。该方法具有筛选速度快、用药量少、漏筛率小等特点 ,比较适合于新杀螨剂创制研究中对大量新化合物杀螨活性的初步筛选

【Abstract】 A method of acaricidal microscreening was introduced. In this method a bean leaf(=2 cm) was placed over the absorbent cotton at the center of plastic culture dish (=9 cm). And then 10 female adult mites were put on the bean leaf to lay eggs and reared. Then the prepared various stage mites were used to spray acaricides with Potter spray tower, and culture and observe the mites in prescriptive room. This method possesses the excellent character in terms of less dosage and high efficiency of biological screening. It is propitious to the preliminary screening of acaricidal activity to substantial novel compounds.

【关键词】 叶螨; 杀螨剂; 叶碟喷雾法;
【Key words】 spidermite( Tetranychus spp.); acaricide; leaf disc spray;
【基金】 国家南方农药创制中心“九五”科技重点项目 (No.96 - A0 2 - 0 4 - 0 2 )
  • 【文献出处】 农药学学报 ,Chinese Journal of Pesticide Science , 编辑部邮箱 ,2002年03期
  • 【分类号】S482.52
  • 【被引频次】5
  • 【下载频次】130
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