【摘要】 以甘薯品种“栗子香”的胚性悬浮细胞为材料,用PEG6000和NaCl分别作为耐旱性和耐盐性选择剂,对甘薯耐旱、耐盐突变体离体筛选的适宜选择压和筛选方法进行研究。①将“栗子香”的胚性悬浮细胞分别培养在含有0～35％PEG和2.0mg/L2,4-D的MS液体培养基中,结果表明,30％PEG为甘薯耐旱性离体筛选的适宜选择压。采用多步选择法和离体重复筛选,由经过γ-射线80Gy照射的900个“栗子香”胚性细胞团筛选得到17个抗性愈伤组织,其中16个再生出植株。②将“栗子香”的胚性悬浮细胞培养在含有0～3.0％NaCl和2.0mg/L2,4-D的MS液体培养基中,结果表明,2.0％NaCl适合甘薯耐盐性离体筛选。采用多步选择法,由900个辐照后的胚性细胞团获得22个抗性愈伤组织,其中20个再生出植株。初步离体鉴定结果表明,这些再生植株比对照表现出较强的NaCl耐受性。更多还原

【Abstract】 In vitro selection of drought- and salt-tolerant mutants in sweetpotato (cv. Lizixiang)was studied by using PEG 6000 and NaCl as selection stress of drought-and salt-tolerances,respectively.①Embryogenic suspension cultures of Lizixiang were cultured in MS media containing 0~35% PEG and 2.0 mg/L 2,4-D.The results showed that 30% PEG could be used as the optimal selection stress of drought-tolerance.Using a multistep selection procedure and repeated in vitro selection, 17 drought-tolerant calli were obtained from the 900 cell-aggregates of Lizixiang irradiated with 80 Gy γ-ray, 16 of them regenerated plants.②Embryogenic suspension cultures of Lizixiang were cultured in MS media containing 0~3.0% NaCl and 2.0 mg/L 2,4-D.The results showed that 2.0% NaCl could be used as the optimal selection stress of salt-tolerance. Using a multistep selection procedure, 22 salt-tolerant calli were obtained from the 900 cell-aggregates of Lizixiang irradiated with 80Gy γ-ray,20 of them formed plants. In vitro identification showed that these regenerated plants were more tolerant to NaCl than the control plants.更多还原