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应用光生物反应器高密度培养等鞭藻
Study on Culturing High-density Isochrysis galbana in Photobioreactor
【摘要】 在经济型 10L气升式光生物反应器上进行了等鞭藻 (Isochrysisgalbana)高密度培养的研究 .结果表明 :一次性培养 12d ,藻细胞由最初的接种密度 1.9× 10 5/mL ,9d后上升到最高值 1.4 1× 10 7/mL ;定时补充营养盐试验过程中 ,经过 12d培养 ,藻细胞密度由最初接种密度 1.8× 10 5/mL上升到最高值 6.0 5× 10 7/mL ,是一次性培养试验相同接种密度、相同培养时间内最高密度 1.4 1× 10 7/mL的4 .2 9倍 .定时补充营养盐试验的第 12天 ,放去 2 /3体积的藻液 ,加回新鲜培养液继续培养 ,2d后 ,藻细胞密度由 1.5 6× 10 7/mL升高到 3.5× 10 7/mL ,这对于快速培养饵料生物具有重要意义
【Abstract】 The high-density cultures of Isochrysis galbana were carried out in a 10 L airlift ecnomical algal photobioreactor. The result shows:cell density increased from 1.9×10 5/mL to 1.41×10 7/mL after 12 days′ batch culture. Cell density of enriched culture increased from 1.8×10 5/mL to 6.05×10 7/mL which was 4.29 times more than the result of batchexperiment in enriched culture for 12 days′cultivating in the second stage of enriched culture, 2/3 v algal liquid was taken out of photobioreactor and new liquid added, its inoculative density of which was 1.56×10 7/mL which was like high-density cultivation. The density reached 3.50×10 7/mL after two days. This would be significant for quickly cultivating food-biology.
- 【文献出处】 宁波大学学报(理工版) ,Journal of Ningbo University(Natural Science & Engineering Edition) , 编辑部邮箱 ,2002年03期
- 【分类号】S968.4
- 【被引频次】12
- 【下载频次】201