【摘要】 探讨纤维蛋白原Bβ基因启动区的两个多态性位点Bβ 14 8C T和 4 5 5G A与冠心病的相关关系。用聚合酶链反应—限制片长多态性方法对冠心病组 (14 8例 )和健康对照组 (173例 )进行了研究 ,发现Bβ 14 8T和 4 5 5A等位基因频率在冠心病组中高于对照组 (P <0 .0 5 ) , 14 8CT及 4 5 5GA基因型频率在冠心病组高于对照组 (P <0 .0 5 ) ,不同基因型血浆纤维蛋白原水平有差异 , 14 8CC和 4 5 5GG < 14 8CT和 4 5 5GA < 14 8TT和 4 5 5AA。冠心病患者组血浆纤维蛋白原水平高于对照组 (P <0 .0 1) ,两组人群同一基因型比较 ,冠心病患者组纤维蛋白原水平均高于健康对照组。表明纤维蛋白原Bβ基因启动区 14 8C T和 4 5 5G A多态性与冠心病的发生相关 ,并可影响血浆纤维蛋白原水平 ;在中国南方人群中纤维蛋白原Bβ 14 8C T和Bβ 4 5 5G A多态性位点呈完全连锁不平衡关系更多还原

【Abstract】 Aim To investigate the relationship between the promoter region of Bβ fibrinogen (Fg) gene polymorphism 148C/T, 455G/A and coronary heart disease (CHD). Methods By Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) restriction enzyme digestion methods,the genotypes and allele frequencies of Bβ fibrinogen gene polymorphism 148C/T, 455G/A were determined in 148 CHD patients and 173 controls. Result Fg Bβ 148T, 455A allele frequencies are higher in CHD group than that in control group (P<0.05). Both heterozygous genotype frequencies of 148CT and 455GA are higher in CHD group than that in control group. The plasma Fg levels have differences among genotypes: 148CC, 455GG< 148CT, 455GA< 148TT, 455AA respectively. The plasma Fg levels are higher in CHD group than that in control group, when compared the same genotype, CHD group Fg levels also higher than that of in control group. Conclusion The promoter region of Bβ Fg gene polymorphism 148C/T, 455G/A are associated with the development of CHD, and at same time impact on the plasma Fg concentration. The polymorphism 148C/T and 455G/A are complete linkage disequilibrium in Southern Chinese.更多还原