【摘要】 探讨山茱萸总苷抗类风湿关节炎的作用机理。采用改良的Boyden小室前导法检测CⅡ诱导的关节炎大鼠山茱萸总苷治疗组和模型组的原代滑膜细胞培养上清对关节浸润炎性细胞如中性粒细胞、骨髓巨噬细胞和淋巴细胞趋化能力,结果以实验组穿膜细胞数减去对照组穿膜细胞数表示。结果发现:山茱萸总苷治疗组的原代滑膜细胞培养上清对上述三种炎性细胞的趋化能力（穿膜细胞数）均显著低于模型组（P<0.01）。说明山茱萸总苷抑制关节滑膜细胞对炎性细胞的趋化,减少炎性细胞向关节滑膜的浸润是其抗关节炎作用的机理之一。更多还原

【Abstract】 This experiment was designed to investigate the mechanism against rheumatoid arthritis of the total glycoside from the dried sarcocarp of Cornus officinalis (TGCO). Modified Boyden chamber technique was used to assay chemotaxis of the supernatant of cultured primary synoviocytes of the rats with rheumatoid arthritis induced by C Ⅱto joint-invading inflammatory cells such as neutrophils, marrow-derived macrophages and lymphocytes. The results were expressed by the difference between the numbers of membrane-penetrating cells in the TGCO-treating group and that in the control group. The results showed that chemotaxis of the supernatant of cultured primary synoviocytes treated by TGCO was much weaker to the above-mentioned three kinds of cells, compared with that of the control group (P < 0.01), which suggests that TGCO can inhibit chemotaxis of the articular synoviocytes to the inflammatory cells, and reducing the number of inflammatory cells in articular synovium is one of the mechanisms of TGCO against rheumatoid arthritis.更多还原