【摘要】 以毛叶枣优良品种“高朗 1号”成年树的茎段为外植体 ,研究了毛叶枣的离体繁殖技术。结果表明 :在附加NAA 0 .1mg/L、BA 2 .0mg/L或另加KT 0 .5mg/L的MS培养基上可诱导腋芽高频率萌发 ;试管芽苗在分别附加NAA 0 .1mg/L与BA 1mg/L、IAA 1～ 2mg/L与BA 1mg/L的MS增殖培养基上 ,增殖系数均达 5以上 ,并且芽苗长势旺盛 ;采用瓶外生根技术 ,无根试管苗移栽成活率 85 %以上。试管苗移植大田后生长正常 ,即将开花结果更多还原

【Abstract】 The micropropagation technique was developed from the explants of stem nodes with axillary buds of the full-grown trees of Zizyphus mauritiana Lam cv Gaolang No1. The results showed that the axillary bud germination at high frequency was obtained on MS media supplemented with NAA 0.1 mg/L, BA 2.0 mg/L or together with KT 0.5 mg/L. The proliferation coefficients of the shoots were up to 5 and the shoots grew well on the proliferation MS media with NAA 0.1 mg/L and BA 1mg/L or IAA 1～2 mg/L and BA1 mg/L. The proliferated shoots directly transferred from culturing containers to the seedling beds could survive at a rate of over 85%. After transplanted to the field, the seedlings grew normally and flowered and bore fruit soon.更多还原