【摘要】 目的 :探讨食管癌相关基因p5 3和PCNA改变与患者生存期的关系。方法 :本研究以 114例高发区食管癌患者为研究对象 ,采用组织病理学和免疫组织化学等方法检测p5 3和PCNA基因在食管癌组织中的表达状况 ,结合患者的临床资料 ,肿瘤分期和组织病理学分级 ,分析发生不同分子变化的食管癌患者的生存风险 ,揭示p5 3和PCNA与食管癌预后的关系。结果 :p5 3高表达与低表达组间无显著差异 ;PCNA高表达患者生存期小于低表达患者 ;Cox回归模型多因素分析结果显示 ,TNM分期、分化程度和年龄对患者生存期的影响较大 ,且有显著性 (P <0 .0 5 )。结论 :p5 3和PCNA不能作为独立的食管癌预后因子 ,而TNM分期仍然是最重要的食管癌预后指标。更多还原

【Abstract】 Objective: To further characterize the relationship between p53 and PCNA and the survival time of esophageal carcinoma (EC) patients.Methods: The present study was undertaken to detect the changes of p53 and PCNA in 114 samples of esophageal carcinoma from high-incidence area, using immunostaining and histopathological methods. Multivariate analysis was performed to evaluate the relationship between the prognosis of EC and nine variables, including TNM, histopathological grades, and et al.Results: The results showed that there was no significant difference between high- and low-expression of p53; patients with high-expression of PCNA had longer survival time than those with low-expression. The result of multivariate analysis by Cox model showed TNM, differentiation and age were sighificahtly correlated with the survival time of EC patients(p<0.05).Conclusion: That p53 and PCNA were not independent prognosis factor of EC, while TNM stages still played an important role in evaluating the survival time of EC patients.更多还原