【摘要】 目的 :为了确定我国汉族人群遗传多态性片段大小范围与法国人群的差异 ,更好的进行基因组扫描和个体识别等方面研究。方法 :4 0 0个微卫星位点分别在 32个反应管中进行扩增。在 5 μl反应体系中加 6～ 17对PCR引物 ,多个微卫星位点同时扩增 ,产物在ABI377XLDNA测序仪上进行电泳。结果 :4 0 0个微卫星位点共有 30 6个有满意的结果 ,其中 12 4个微卫星位点与法国CEPH家系存在片段范围的差异。结论 :遗传多态性片段大小范围在种族间存在较大差异 ;在对中国汉族人群进行基因分型时 ,应在Genethon提供的范围基础上向两侧各扩大 10bp。更多还原

【Abstract】 Objective To facilitate the application of the large scale genome scan and individual identification by investigating the differences of microsatellite polymorphisms of the genethon human genetic linkage map between the Han nationality and French population. Methods Six to seventeen pairs of primers were added in one single tube with 5 μl reaction volume and several microsatellite markers were amplified simultaneously. The products were electrophoresited on the 377XL DNA sequencer. Results During the entire experiment 400 microsatellite loci were amplified in 32 tubes and 306 microsatellite loci were satisfactory.Conclusion The size range of genetic polymorphisms are different in race, and the difference exists at 124 microsatellite loci between the Han nationality and French population, so 10 bp should be added to both sides of the genethon map size range when we perform genotype in Han nationality.更多还原