【摘要】 摘要 取24只小鼠分为慢性热应激组(EGⅠ)、急性热应激组(EGⅡ)和空白对照组(CG)。EGⅠ组每天早上移入40℃(相对湿度为53％～56％)的热应激箱内热应激10min,连续9d,到第10d,EGⅠ和EGⅡ同上热应激15min。结果显示:在热环境下,EGⅠ、EGⅡ组动物的肛温均比CG组高,EGⅡ组又比EGⅠ组高;3组动物睾丸重量及指数无明显变化;EGⅠ、EGⅡ两组动物精子数量及活动率均比CG组明显低(P<0.01);畸形率比CG组均高,EGⅠ组的精子数量、活动率显著低于EGⅡ组,畸形率极显著地高于EGⅡ组(P<0.01);在畸形的精子中,CG组主要以头部缺失为主,而EGⅠ组却以尾部缺失为主,EGⅡ组头部、尾部缺失相近。结果表明:热应激对精子的发生过程有毒性作用,抑制精子的形成,降低精子的数量及活动性能。更多还原

【Abstract】 To study the effect of heat stress(HS) on spermatogenesis and spemi capacity in mice. 24 male KM mice were divided into chronic HS group(EG Ⅰ ) .acute HS group(EGⅡ )and control group(CG). EG Ⅰwas placed in the box at 40℃ and relative humidity 53-56% for10 min every day, it last 9 days. EG Ⅰand EG Ⅱ were exposed in the same box for 15 min on tenth day. The anal temperature of mice in EG Ⅰ and EG Ⅱ were significantly increased under above thermal environment. The weight and index of testes( epiditymis ) was no change remarkly. The number, activity rate of sperm of EG I and EG Ⅱ were lower than that of CG( P< 0.05) .However, sperm malformation rate of EGⅠshowed obviously increased. The number, activity rate of sperm of EG Ⅰ obviously decreased, but malformation rate of EG Ⅰobviously increased compared with EG Ⅱ (P<0.01). In the malformation sperms, malformation sperm of CG was mainly loss- head, that of EG I was mainly loss- tail and that of EGⅡ was equivalence. Heat stress damaged spermatogenesis, inhibited sperm formation , decreased sperm number and sperm capacity.更多还原