节点文献
一种改进的昆虫基因组DNA的提取方法
An Improved Method for Extracting Genomic DNA of Insect
【摘要】 就昆虫总DNA的提取 ,介绍了一种简便、快速的提取方法 ,此方法既可对新鲜标本进行DNA提取、也可对冷冻标本、干标本、酒精泡制标本进行DNA提取 ,均能得到较完整的大分子DNA片段 ,并且得率较高 .改进的昆虫总DNA提取方法简便、快速 ,对设备和试剂的要求都不高 .
【Abstract】 The technique of molecular biology has been widely applied in the field of insect taxonomy. The most important is the insect DNA extraction. The paper represents an improved method for extracting the genomic DNA from both fresh samples and freeze sample. Its advantages are simplicity, cheapness and lower requirement for equipment and materials.
【基金】 国家自然科学基金重大资助项目 (30 130 0 4 0 )
- 【文献出处】 河北大学学报(自然科学版) ,Journal of Hebei University(Natural Science Edition) , 编辑部邮箱 ,2002年01期
- 【分类号】Q781;Q969
- 【被引频次】79
- 【下载频次】985