【摘要】 通过六因素三水平正交实验研究了几种不同类型的营养物质和前体对东北红豆杉生产紫杉醇的影响。结果表明,在第12d加入lmmol·L-1苯丙氨酸,2mg·L-1赤霉素,1mmol·L-1乙酸钠,100mmol·L-1甘露醇,0.1mmol·L-1苯甲酸钠和2mmol·L-1L-谷氨酰胺。可以使紫杉醇产量增加535.2％,而且各种营养物和前体均不同程度地促进了紫杉醇的合成。更多还原

【Abstract】 The promoting effect of some different nutrients and some possible precursors on taxol biosynthesis has been studied through the orthogonal tests, the results show that the addition of 1mmol·L-1 phenylalanine,0. 1mmol·L-1benzoate, 1mmol·L-1acetate,2mmol· L-1-glutamine, 2mg·L-1GA3,100mmol ·L-1mannite fructose into the cultures on the 12thday of culture dramatically increased taxol yield by 535. 2%. The results also demonstrated that taxol biosynthesis was improved in different degrees by nutrient/precursor.更多还原