【摘要】 本文是在三步法合成用于时间分辨激光荧光免疫分析的 4 ,7 二 (氯磺酰基苯基 ) 1,10 菲罗啉 2 ,9 二羧酸 (BCPDA)基础上 ,为提高产物纯度 ,探讨五步合成方法。本法经氯化、酯化、制备二钠盐、还原二羧酸、磺化 ,获得了目标产物———BCPDA。研究了中间产物重结晶条件和结构参数。并将五步合成目标产物与三步合成的目标产物的结果通过熔点、红外光谱、核磁共振氢谱、元素分析及其某些物理、化学性质进行了报道。与国外文献结果具有较好的一致性 ,正在进行的标记分析实验证实了BCPDA的双重功能更多还原

【Abstract】 In this paper,we report on a solid phase time resolved fluorescence immunoassay chelate reagent 4,7 bis(chlorosulfophenyl) 1,10 phenanthroline 2,9 dicarboxylic acid (BCPDA),which is suitable as a fluorescent labeling agent.The five step synthesis product of BCPDA was presented for improving the purity of the product based on the three step synthesis product.The approach involves chlorization,hydrolyzing the ester,preparing disodium,carboxylate to diacid,sulfonation.The yield of five step product is 99%,45%,94%,95%,80% respectively.The structure and purity of product was characterized by the melting point,IR, 1H NMR,UV spectrum,element analysis,and proved to be consistent with the structure predictal.更多还原