【摘要】 用快速脂肪提取器对雄、雌虾蛄肉及虾蛄头中的脂肪进行了提取和测定。脂肪经KOH CH3OH溶液水解并甲酯化后 ,采用 60m× 0 .3 2mmi.d.交联PEG 2 0M弹性石英毛细管柱 ,FID检测器 ,结合GC MS ,对脂肪酸进行了分析。结果表明 :雄虾蛄肉脂肪含量为 5 .1 4 % ,不饱和脂肪酸占脂肪酸总量的 74 .7% ,其中多不饱和脂肪酸 (PUFA)为 4 9.3 % ,EPA +DHA为 3 0 .2 % ;雌虾蛄肉脂肪含量为 6.0 2 % ,不饱和脂肪酸占脂肪酸总量的 71 .5 % ,其中多不饱和脂肪酸 (PUFA)为 4 2 .1 % ,EPA +DHA为 2 5 .2 % ;虾蛄头脂肪含量为 5 .65 % ,不饱和脂肪酸占脂肪酸总量的 74 .3 % ,其中多不饱和脂肪酸 (PUFA)为 5 1 .5 % ,EPA +DHA为 2 9.7%。更多还原

【Abstract】 Fat in male and female squillid meat and squillid heads was extracted with diethyl ether by means of rapid fat extractor instead of Soxhlet extractor respectively. Fat was dissolved with 1mL of benzene and diethyl ether( V/V ,1∶1),hydrolyzed and esterified with potassium hydroxide and methanol.After centrifugation,fatty acid esters in upper benzene and ether layer were separated and determined by a 60 m×0.32 mm i.d. fused silica capillary column coated with cross-linked PEG-20M and determined by hydrogen flame ionization detector. The injector and detector temperature was 250℃, the column temperature was programmed from 170℃ to 175℃ at 5℃/min (4 min), and then from 175℃ to 190℃ at 10℃/min. Forty-five peaks were identified by gas chromatography-mass specrometry. C 16∶0 is the predominat saturated fatty acid. C 16∶1 is the most abundant monunsaterated fatty acid. C 18∶4 , C 20∶5 and C 22∶6 are predominat polyunsaturated fatty acids. The contents of unsaturated fatty acids (UFA) in male and female squillid meat and squillid heads were 74.7%, 71.5% and 74.3%, of which, the contents of polyunsaturated fatty acids were 49.3%, 42.1% and 51.5%, the contents of eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) were 30.2%, 25.2% and 29.7%, respectively.更多还原