【摘要】 目的对比观察正常培养皮层神经元在N-甲基-D-天冬氨酸(NMDA)及其受体拮抗剂—MK-801作用下膜表面三维构像形态的改变。方法利用分辨率为0.1~0.01nm的原子力显微镜(AFM)对原代培养大鼠皮层神经细胞膜表面进行纳米尺度的扫描观测。结果正常神经元膜表面光滑,起伏均匀,隆起的颗粒状蛋白密集,间隔规律;NMDA损伤后神经元破碎,崩解,膜失去连续性;NMDA+MK-801作用下神经元膜皱折增加,边缘粗糙,起伏程度介于前两者之间。结论(1)AFM具有分辨率高,制样简单特点。(2)AFM能细微地分辨损伤保护作用后引起的细胞膜表面三维形态改变。(3)NMDA作用后膜结构开始解体,膜蛋白颗粒聚集增大,脂质凹陷加深,间距增宽,表面粗糙度增加。更多还原

【Abstract】 Objective To observe the changes in three-dimensional morpholog y of the membrane of the cortex neurons in pri-mary culture in response to treatm ent with N-Methyl-D-Asp (NMDA) and MK-801 using atomic force microscope (AFM). Methods Following NMDA treatment, the changes in the membrane of the neurons fix ed by glutaraldehyhyde were examined, and the protective effect of MK-801 on th e neurons was also observed using an ATM at the resolution of 0.01-0.1 nm. Resul ts Normal neurons presented smooth membrane surface with regular undulation and densely but well arrayed protein granules. In response to NMDA treatment, the n eurons were disrupted, falling into small pieces, and their membrane appeared di scontinu-ous. MK-801 treatment increased the folds of the membrane that showed r ough edges, with membrane undulation only sec-ondary to normal condition. Conclu sions AFM, with the merits of high resolution and easy sample preparation, can f inely dis-play the three-dimensional morphology of the surface of the neurons, w hich, after NMDA treatment, becomes disintegrated with increased bulk of the pr otein granules, deepened caving of the lipids, and rougher membrane surface.更多还原