【摘要】 目的 :研究γδT细胞对 EB病毒转化前后人类 B淋巴细胞识别的差异 ,寻找γδT细胞识别的肿瘤 /病毒感染诱生性抗原分子。 方法 :流式细胞仪检测 HSP6 0和 HSP70的表达 ,MTT法检测γδT细胞的杀伤作用 ,RT- PCR分析 MHC- 类链相关基因 A(MHC class chain- related gene A,M CA)的表达。结果 :HSP6 0和 HSP70分子以及 M CA基因在 EB病毒转化后细胞中高表达 ,γδT细胞对转化后细胞杀伤率明显增高 ,且其细胞毒作用能部分被抗 HSP6 0和 HSP70抗体所阻断。结论 :HSP6 0和 HSP70是 γδT细胞对 EB病毒转化后的 B淋巴细胞的识别靶点 ,但尚有其他抗原识别靶点 ,其中 M CA的可能性较大。更多还原

【Abstract】 Objective:To observe the difference of γδT lymphocytes recognition between B lymphocytes and EB virus transformed B cells,identifying antigens recognized by γδT lymphocytes.Methods:Expression of HSP60 and HSP70 on the surface of B cells were detected by flow cytometry.MTT method was used to detect the cytotoxicities of γδT lymphocytes.RT PCR was used to detect expression of MⅠCA gene.Results:Compared to normal control,almost all EB virus transformed B lymphocytes expressed HSP60,HSP70 and MⅠCA gene.The cytotoxicity of γδT lymphocytes to EB virus transformed B lymphocytes was partly blocked by combining anti HSP60 antibody and anti HSP70 antibody.Conclusion:Two antigens of EB virus transformed B lymphocytes recognized by γδT lymphocytes are HSP60 and HSP70,which may be partly related to cytotoxicity of γδT cells.There may be other EB virus transformed related antigens as ligands for TCRγδ,MⅠCA is an important candidate.更多还原