【摘要】 目的 :了解Shh(Sonichedgehog) ,Bmp - 4,Ptc(patched)基因在角化囊肿中的表达情况 ,明确Shh及其相关基因在角化囊肿发病中的作用。方法 :选取角化囊肿石蜡切片。将Shh ,Bmp 4,Ptc分别转染与大肠杆菌XL 1 Blue ,提质粒 ,线性化质粒 ,体外转录法合成地高辛标记的RNA探针 ,原位杂交检测角化囊肿中Shh ,Bmp 4,Ptc的表达情况。结果 :Shh ,Bmp 4共同表达于角化囊肿上皮细胞 ,胞浆呈强阳性表达 ;Ptc表达于角化囊肿间充质细胞 ,胞浆表达较弱。结论 :Shh ,Bmp 4,Ptc在角化囊肿中都有异常表达 ,提示 (1)角化囊肿的发生与生长发育中的上皮残余有关 ;(2 )Shh与Ptc之间平衡的破坏是发生角化囊肿的条件之一 ;(3)角化囊肿与正常生长发育相似 ,存在Bmp 4与Shh协同表达更多还原

【Abstract】 Objective: To understand effect of sonic hedg ehog Shh,and its related genes in keratocysts by observing expression of Shh, Bmp-4 and Ptc. Methods: Paraffin-embedded sections of keratocyst were selected from Pathological Department, Peking University Stomatological School. Vectors containing fragments of Shh, Bmp-4 and Ptc were transfected into XL-1-blue, vectors were harvested and transcripted in vitro to synthesize the Dig-labeling RNA probes. The gene expression of Shh, Bmp-4, Ptc were detected in the keratocyst by in situ hybridization. Results: Shh and Bmp-4 expressed strongly in the epithelial cells, while Ptc expressed weakly in the mesenchyme. Conclusion: Shh, Bmp-4 and Ptc express abnormally in keratocysts. The result suggested that (1)Shh and related genes play important roles in keratocysts; (2)Epithelium remnants are related to the formation of keratocysts; (3)Bmp-4 and Shh co-express pathologically in the epithelium of keratocysts, resembling the normal developmental progress.更多还原