【摘要】 目的 :研究肠道非霍奇金恶性淋巴瘤的 EB病毒感染、p5 3、p2 1ras蛋白表达及其相关性。方法 :应用单克隆抗体 CD4 5RO、CD2 0 、CD79a、CD4 5RA、CD56 、L CA、CD30 、CD1 5、CD5、CD8、TIA- 1、Gram B、p5 3、p2 1;多克隆抗体 CD3、κ、λ对瘤细胞进行免疫组化标记 ,用 SABC免疫组化的方法检测瘤细胞 p5 3、p2 1ras基因的表达及 EB病毒寡核苷酸探针 (EBER)原位杂交 ,观察了 4 0例 NHL 的免疫表型、EBV感染情况及 p5 3、p2 1ras蛋白的表达。结果 :4 0例中 ,经免疫组化证实 6例为 T细胞淋巴瘤(15 % ) ,34例为 B细胞淋巴瘤 (85 % )。T细胞淋巴瘤为外周 T细胞性和肠病型 T细胞性淋巴瘤。B细胞淋巴瘤 9例为弥漫性大细胞性淋巴瘤 2 3.5 % ,2 5例为边缘区 B细胞性淋巴瘤 (76 .5 % )。EBV-ERBR原位杂交 6 / 4 0例有阳性表达 ,均为 T细胞淋巴瘤 ,阳性细胞占肿瘤细胞的 30 %～ 80 %。 p5 3的表达共有 2 5例 ,2 1例有 p2 1ras的表达。有 16例同时检出 p5 3和 p2 1ras的表达。结论 :肠道淋巴瘤以边缘区 B细胞淋巴瘤多发临床为惰性。如为 T细胞性淋巴瘤 ,更多见的是侵袭性 ,且 T细胞淋巴瘤与 EBV相关性较高 B细胞淋巴瘤则无相关性。p5 3的表达与 EBV感染无明显相关性 ,而 p2 1ras的表达与 EBV感染似有关系。更多还原

【Abstract】 Objective:To study the relationship of Epstein Barr virus in fection,p53 and p21 ras expression in non Hodgkin′s lymphoma of intestine.Methods:Immunohistochemistry studies were performed using monoclonal and polyclone antibodies:CD 45RO ,CD 3 for T lymphocyte;CD 20 ,CD 79a ,CD 45RA and κ,λ for B lymphocyte;CD 56 for NK cell,Gram B and TIA 1 for NK cell and cytotoxic T cell.p53 and p21 ras for tumor cells.In situ hybridization for EBV encoded small nuclear RNA (EBER).Results:Of 40 cases,6 and 34 cases were confimed as T and B cell lymphomas,respectively,using immunohistochemistry method.The T cell lymphoma cases are peripheral T and intestinal T cell lymphomas.The B cell lymphoma are diffuse large B cell,marginal B cell lymphomas (25/40 cases,MALT type).EBV EBER in situ hybridization positive in 6/40 cases,all of which were T cell type,and there are 30 %～80 % positive tumor cells among them.p53 expressed in 25 of 40 cases and p21 ras expressed in 21 cases.Both p53 and p21 ras expressed in 16 cases.Conclusion:The lymphomas of intestine are more often as marginal zone B cell type and most of them are indolent,which suggests that T cell lymphoma always means invasive.The results also show that T cell lymphoma has high relationship with EBV infection.The expression of p53 don′t have obvious relationship with EBV,but p21 expression seems to have some relationship with it.更多还原