【摘要】 目的 :研究清开灵注射液及其有效成分治疗急性白血病的作用机理。方法 :采用体外培养技术、MTT法、细胞形态学、DNA凝胶电泳及流式细胞检测技术 ,对清开灵及其有效成分诱导的人急性早幼粒白血病 (HL 60 )细胞凋亡进行分析。结果 :清开灵及其有效成分黄芩甙、猪去氧胆酸对HL 60细胞均有很强的细胞毒作用 ,牛黄胆酸的作用次之 ;前三者在体外作用 6h即可诱导细胞凋亡 ,流式细胞仪检测出现典型凋亡峰 ;牛黄胆酸无诱导HL 60细胞凋亡作用。结论 :清开灵注射液及其有效成分可在体外诱导白血病细胞凋亡 ,可能是其治疗急性白血病的作用机理之一。更多还原

【Abstract】 Objective: To investigate the mechanism of Qingkailing (QKL) Injection in treating acute promyelocytic leukemia. Methods: Using MTT technique, cell morphologic method, DNA gel electrophoresis and flow cytometry to study the human acute promyelocytic leukemia (HL 60) cell apoptosis induced by QKL and its active principle. Results: QKL and its active principle, Baicalin and hyodeoxycholic acid, showed strong cytotoxicity in inhibiting HL 60 cell, the Bezoar cholic acid showed a weaker effect. Apoptosis could be induced after being treated for 6 hrs by the former three principles, showing a typical apoptosis peak under flow cytometry, but could not be induced by the latter one. Conclusion: QKL could induce leukemia cell apoptosis in vitro, which may be one of the mechanisms of QKLI in curing acute promyelocytic leukemia.更多还原