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As1.398水解菜籽蛋白的酶水解条件的研究
Study on Hydrolytic Conditions of Rape-seed Protein by As1.398
【摘要】 用响应面分析法 (RSM)对影响菜籽蛋白的酶水解的因素进行分析 ,得到As1 398的最佳酶水解条件为温度 4 9 6℃ ,pH7 6,酶与底物浓度比 (E/S) 74 2 0PU/g ,底物浓度 9 4 % (w/v) ,水解时间 6h ,最佳酶水解条件下的水解度为 16 2 %。在最佳酶水解条件下对水解度和时间的关系进行了研究 ,证实了预测值和实测值是一致的。
【Abstract】 Response surface methodology(RSM) was used to optimize the hydrolytic conditions,which for As1 398 were:49 6℃,pH7 6,enzyme/substrate ratio of 7 420 (PU/g),Substrate/solvent ratio of 9 4%(w/v),reaction time of 6 h.Under the optimum conditions,the relationship between the degree of hydrolysis(DH) and time was studied.The result proved that the predicative value was consistent with the test value.
- 【文献出处】 中国油脂 ,China Oils and Fats , 编辑部邮箱 ,2001年01期
- 【分类号】TQ936
- 【被引频次】16
- 【下载频次】95