【摘要】 目的 :观察调心方对由TNFα加Aβ(2 5～ 35 )诱导的SK N SH (神经源性细胞株 )细胞毒性的保护作用。 方法 :用TNFα 5 0ng/ml和Aβ(2 5～ 35 ) 0 1μM处理SK N SH细胞造成细胞损伤模型。以MTT和DNAladder为指标。 结果 :TNFα和Aβ(2 5～ 35 )处理SK N SH细胞 2 4小时细胞生存率减少约 5 0 % ,而调心方含药血清能提高细胞模型的生存率 ,减少细胞DNA的降解。结论 :一定剂量的TNFα加小剂量的Aβ(2 5～ 35 )能造成接近体内病理生理机制的细胞损伤模型 ,调心方对TNFα加Aβ(2 5～35 )造成的细胞毒性具有一定的保护作用更多还原

【Abstract】 The effects of Heart Benefiting Receipt (HBR) on Aβ(25～35) plus TNFα induced toxicity was investigated in human neuroblastoma cell line SK N SH by measuring the mitochondria activity and DNA laddering. Treatment of SK N SH cells for 24 hours with TNFα 50ng/ml plus Aβ (25～35) 0.1μM resulted in a significant inhibition on MTT reduction (50%) when the normal control was defined as 100%. HBR was found in clinical relevant concentration to attenuate TNFα 50ng/ml plus Aβ (25～35) 0.1μM induced toxicity in SK N SH cells and DNA degradation. This study demonstrates that TNFα 50ng/ml plus Aβ (25～35) 0.1μM can mimic patho physiological condition which might happen in patients with Alzheimer’s disease, and HBR can exert neuroprotective properties which might be of importance and contribute to cliniccal efficacy to treatment of Alzheimer’s Disease.更多还原