【摘要】 目的 获得可用于动物试验和临床试验的人表皮生长因子原料药。方法 化学合成的人表皮生长因子基因在甲基营养型酵母中表达并分泌到培养基中的人表皮生长因子蛋白经 Phenysepharose 6 Fast Flow（ high sub）柱、 Q-sepharose High Performance柱、 Superdex 30柱纯化后进行生物学性质检测。结果 纯化后的蛋白产物纯度达 98％，是无热源、无内毒素、无甲基营养型酵母染色体 DNA污染的，有正确的分子量、等电点、氨基末端氨基酸顺序、肽图、紫外光谱、及生物活性性质的重组蛋白。结论 纯化蛋白符合动物试验和临床试验的要求，为制备多种人表皮生长因子制剂进行动物实验和临床试验打下了基础。更多还原

【Abstract】 Objective To obtain recombinant human epidermal growth factor(hEGF) that can be used in animal experiments and clinical trial. Method Chemically synthesized hEGF gene was expressed in Yeast Pichia pastoris and the secretory hEGF was purified by Phenysepharose 6 Fast Flow（ high sub） ,Q-sepharose High Performance ,and Superdex 30 chromatography， and its characters were studied by respective methods . Results The purified hEGF doesn’t contain pyrogen, endotoxin, or yeast chromosome DNA and the purity reached 98％ .The recombinant human EGF has correct molecular weight , pI, N-terminal amino acids sequences ,peptide map ,ultraviolet spectrum and well-biological activity. Conclusion The purified hEGF is in accord with the requirements for animal experiments and clinical trial which provids the basis of preparing EGF agents for clinical test.更多还原