【摘要】 目的:本实验观察当归补血汤对低渗引起的血管内皮通透性增加的抑制作用。方法:从新生牛主动脉分离获得单个内皮细胞,培养于微孔滤膜上直至形成致密单层。灌流含5g·L^-1白蛋白的hanks液后,测定经低渗溶液处理（将M 199溶液中含的20％血清改成2％血清）或经低渗加10^-4g.ml^-1当归补血汤溶液处理的液体滤过系数（Kf）、液体滤过流量（Jv）和蛋白质渗透压反射系数（σ）。结果:内皮单层经低渗溶液处理后,Kf和Jv降低,σ升高;而当归补血汤能够抑制因低渗造成的Kf和Jv降低,以及σ升高。形态学分析表明当归补血汤能够抑制因低渗造成细胞肿胀和细胞间距离的增宽。结论:低渗能够引起血管内皮单层通透性的增加,而当归补血汤能够抑制这种增加。更多还原

【Abstract】 Objective:To study effect of Radix Angelicae Sinensis decoction for supplementing blood (RASDSB) on inhibiting the increaseof endothelial cell monolayer permeability induced by hypoosmotic soluion. Methods: The endothelial cells isolated from newborn bovine aortawere cultured on polycarbonate microporous filter membrane to develop compact endothelial monolayer. Fluid filtration coefficient (Kf), filtration volume (Jv) and osmotic reflective coefficient (σ) to protein of the endothelial monolayer were measured treated by hypoosmotic solution (changing concentration of serum in M199 solution from 20% to 2%) for 120 min or by hypoosmotoe solution containing 10-4g·ml-1 RASDSB for 120 min after perfused Hanks balanced salt solution containing 5 g·L-1 albumin. Results: Kf and Jv of the endothelial monolayer treated by hypoosmotic solution increased and a of that decreased. RASDSB could inhibit above-mentioned change. Morphological analysis demonstrated that RASDSB could inhibit widening of intercellular distance and enlargement of cellular area in the endothelial monolayer induced by hypoos-molality. Conclusion:Hypoosmotic solution could increase endotehlial cell monolayer permeability and RASDSB could inhibit the increase.更多还原