【摘要】 以耐盐性差的“冀麦 2 4” （TriticumaestivumL .）和其经正定霉素诱变后获得的耐盐突变体 890 1₁7为材料 ,用2 80个引物在两者之间进行RAPD分析 ,其中 35个引物扩增出DNA多态性 ,其相似性系数为 0 .978,证明二者为近等基因系 （near_isogenicline ,NIL）。用分株法建立两个F2 群体 （“冀麦 2 4”× 890 1₁7和 890 1₁7ד中麦 9”） ,在两个群体中按照BSA （bulkedsegregantanalysis）方法分别构建两个对应DNA池 （耐盐池和不耐盐池 ） ,用上述能扩增出明显多态性的 35个引物在对应的耐盐和不耐盐DNA池之间进行RAPD分析 ,发现只有OperonQ4引物在对应的两个DNA池扩增出的多态性在两个F2 群体之间是一致的 ,说明其扩增产物是与耐盐突变位点紧密连锁的RAPD分子标记。更多还原

【Abstract】 Calli from mature embryo of “Jimai₂4” wheat （ Triticum aestivum L.） were induced on medium containing Zhengdingmycin then continuously cultured on medium containing 0.5% NaCl till to regenerate plants named 8901₁7 salt_tolerant mutant. “Jimai₂4” was compared with 8901₁7 by using the technique of RAPD. Thirty_five out of 280 random primers could detect DNA polymorphism. The similarity index was 0.978, indicating that they were NILs （near_isogenic lines）. Two F 2 populations （“Jimai₂4”×8901₁7 and 8901₁7דZhongmai₉”） had been constructed using the method of half_division. The two relative DNA pools （salt tolerant DNA pool and susceptible DNA pool） which come from the two F 2 populations, respectively, had been made according to the method of BSA （bulked segregant analysis）. RAPD analysis between the two DNA relative pools was carried on with above 35 random primers which could detect DNA polymorphism definitely. The identical polymorphism between the two sets of DNA pools come from the two F 2 populations could be determined only by OperonQ4 primer. This result implied that the polymorphic fragment amplified by OperonQ4 primer was the molecular marker of RAPD closely linked to the salt tolerant mutation locus.更多还原