【摘要】 利用水平淀粉凝胶电泳 8种酶 10个位点 34个等位基因 ,对辽宁兴城油松无性系种子园中 17个无性系2 0个单株的 2 0 0粒种子作了父本分析。无性系雄花量的多少与授粉座果率并不成正比 ;不同无性系对花粉的接受存在一定的选择性 ,分析试样单株与周围的无性系并没有构成固定的授粉组合 ,说明授粉期风向等气象因子 ,无性系的雌雄花器的构造 ,受精机理等对受粉的成败都有作用 ;种子园结实初期自交现象并不明显 ,在分析试样中只有16 # ,17# ,19#无性系存在自交现象 ;种子园内花粉的有效传播一般在半径 30 m范围内。同时 ,分析了种子园子代的等位酶变异和空间遗传结构 ,从另一侧面反映出采种母树周围花粉分布比较均匀 ,并说明散粉期间风向等气象因子对授粉成败影响较大更多还原

【Abstract】 Paternity analysis of a clonal seed orchard of Pinus tabulaeformis Carr. was conducted by means of allozyme markers. Multilocus allozyme genotypes at 10 loci of 17 clones were inferred from a sample of at least eight megagametophytes per clone, while the genotypes of the other 32 parental clones were from buds. A total of 200 embryos and endosperms were analyzed, of which the paternity could be accurately identified for just 89 samples. A low degree of selfing was found within the seed orchard, while higher male strobili production did not indicate a higher degree of fertilization success. Half of the allozyme loci were found to deviate from Hardy-Weinberg equilibrium. At the same time, the effective dispersal distance of pollen and spatial genetic structure of the seed orchard population were analyzed by means of spatial autocorrelation. A few Moran’s I values for this test in short distance classes were significantly positive. The results indicated that the pattern of spatial genetic structure is very weak in the progeny population.更多还原